γH2AX: a sensitive molecular marker of DNA damage and repair

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The γH2AX DNA damage assay from a drop of blood

DNA double-strand breaks (DSB) and blocked replication forks activate the DNA damage response (DDR), a signaling pathway marked by phosphorylation of histone 2AX (H2AX). The phosphorylated form, γH2AX, accumulates at the site of damage and can be detected as foci by immunocytochemistry. Therefore, γH2AX is a sensitive and robust biomarker of DNA damage, notably DSB. Cells from peripheral blood ...

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Gadolinium-enhanced cardiac MR exams of human subjects are associated with significant increases in the DNA repair marker 53BP1, but not the damage marker γH2AX

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ژورنال

عنوان ژورنال: Leukemia

سال: 2010

ISSN: 0887-6924,1476-5551

DOI: 10.1038/leu.2010.6